Part:BBa_K3239002

Promoter for the Mit1 transcription factor in the P.pastoris GS115 strain

Promoter for the MIT1 gene in the P. pastoris GS115 strain. Upregulated by Prm1.

Usage and Biology

The MIT1 promoter (P_MIT1) is homogeneous to the P. pastoris GS115 strain. It is upregulated by the Prm1 protein, a transcription factor homogeneous to the P. pastoris GS115 strain.

Functional Parameters

Published Literature has shown that this promoter is tightly regulated by Prm1 and has a very low leakiness.

Reference

Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738

Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014

Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 793
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 793
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 793
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 793
  
• 1000
  COMPATIBLE WITH RFC[1000]